D10.g4.1 mouse helper t cells
WebThe biological activity of recombinant rat IL-1α was measured by its ability to stimulate cell proliferation using a mouse helper T cell line, D10.G4.1. Safety Information. Storage Class Code. 13 - Non Combustible Solids. WGK. WGK 3. Flash Point(F) Not applicable. Flash Point(C) Not applicable. WebRat IL-1 alpha/IL-1F1 in a cell proliferation assay using D10.G4.1 mouse helper T cell. The ED50 range is less than or equal to 20 pg/ml.
D10.g4.1 mouse helper t cells
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WebWe describe here a stable subclone (D10S) of the murine D10.G4.1 helper T-cell which proliferates to subfemtomolar (attomolar) concentrations of IL-1 beta or alpha in the … WebD10.G4.1 (mouse, AKR/J(H-2k), lymphocyte, helper T)SPECIES: mouse, AKR/J(H-2k) TISSUE/ORGAN: lymphocyte, helper T DSMZ catalog code: ACC 45 Morphology: single, round cells in suspension Description: mouse T-helper cells clone established from an AKR/J(H-2 k ) mouse; indicator cell line for IL-1 confirmed as mouse with IEF of AST, …
WebApr 13, 2024 · D10.G4.1 mouse helper T cells in ... costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (TFH) and germinal centers (35, 9, 10). In human but not in mouse, B7H2 additionally binds to CD28 and CTLA4 , and its interaction with CD28 can costimulate both human and mouse naïve ... WebMouse IL-33 induces the proliferation of D10.G4.1 mouse T lymphocytes in a dose dependent manner. Description IL-33 belongs to the IL-1 family and is closely related in …
WebJun 22, 2024 · Briefly, D10.G4.1 cells (2 x 10 6 cells/well) were dispensed into anti-CD3 mAb coated 6-well plates, with or without varying concentrations of NK-4, and incubated for 6 h at 37°C. Total RNA was extracted from D10.G4.1 cells using an RNeasy Mini kit (QIAGEN, Tokyo, Japan) and DNase (QIAGEN) according to the manufacturer’s … WebJun 15, 1987 · After activation with antigen or mitogen, D10.G4.1, an antigen-specific cloned T helper cell that has recently been shown to produce IL 4 but not IL 2, secretes two …
WebATCC mouse d10 g4 1 t helper 2 cell Mouse D10 G4 1 T Helper 2 Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed …
WebRecombinant Mouse IL-1 alpha /IL-1F1 (Catalog # 400-ML) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED 50 for this effect is 3-7 pg/mL. SDS … chinabank filinvest branchWebRecombinant Human IL-1 alpha/IL-1F1 Protein Bioactivity Recombinant Human IL-1 alpha /IL-1F1 (Catalog # 200-LA) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED 50 for this effect is 1-6 pg/mL. Recombinant Human IL-1 alpha/IL-1F1 Protein SDS-PAGE 1 μg/lane of Recombinant Human IL-1 alpha /IL-1F1 … grafana apache proxyWebecombinant human IL-1 beta (HZ-1164) stimulates does-dependent proliferation of the D10.G4.1 mouse helper t lymphocyte cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability … grafana apache echartsWebRight panel: D10.G4.1 (mouse Th2, ATCC TIB -224) cell lysate was probed with Mouse anti-GATA3 monoclonal antibody at concentrations of 0.0625 (lane 1), 0.0156 (lane 2), and 0.0039 µg/ml (lane 3). GATA3 is identified as a band of 50 kDa. MIDDLE LEFT, Immunofluorescent staining of human breast adenocarcinoma. china bank financial statements 2020WebMeasured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED 50 for this effect is typically 2-10 pg/mL. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC 50 of mouse IL1R1 is 0.13 μg/mL. grafana an unexpected error happenedWebED 50 10.0 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, ... keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte ... grafana application insightsWebGeneral. Specific applications. D10.G4.1 has been used extensively to study activation of T cells. These cells can also be used as a reliable and sensitive assay for IL-1 (interleukin-1) response, since D10 cells will … china bank efps payment