Cytochalasin b glucose uptake
WebNov 2, 2011 · Breast cancer cells have a high level of glucose uptake and metabolism, a common characteristic of most cancer cells. This increased glucose uptake and aerobic metabolism forms the basis for assessment of tumor metabolism and response to therapy by positron emission tomography (PET) imaging with FDG (F-18 2-fluoro-2′-deoxyglucose) … WebMay 13, 2024 · A bioluminescent glucose-uptake probe enables accurate, real-time, non-invasive longitudinal imaging of d-glucose absorption both in vitro and in vivo. ... To examine the effect of cytochalasin B ...
Cytochalasin b glucose uptake
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WebA novel phosphoinositolglycan-peptide (PIG-P) from the yeast Saccharomyces cerevisiae potently mimicks insulin action on glucose transport and metabolism in rat muscle and adipose tissue. The aim of the present study was to elucidate the cellular WebJun 18, 2024 · The glucose transporter GLUT1, a plasma membrane protein that mediates glucose homeostasis in mammalian cells, is responsible for constitutive uptake of glucose into many tissues and organs. Many studies have focused on its vital physiological functions and close relationship with diseases.
WebThe widely expressed mammalian glucose transporter, GLUT1, can be acutely activated in L929 fibroblast cells by a variety of conditions, including glucose deprivation, or treatment with various respiration inhibitors.Known thiol reactive compounds including phenylarsine oxide and nitroxyl are the fastest acting stimulators of glucose uptake, implicating … WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. This coincided with a marked decrease in D - [U- 14 C] glucose oxidation and suppression of the stimulant action of D -glucose upon insulin release.
WebNov 10, 2014 · To further confirm our findings in primary brown adipocyte cultures, glucose uptake in BAT was measured in vivo in β 1 /β 2 knockout (to exclude any effects on β 1 - or β 2-adrenoceptors) and wild-type FVB mice that express all three β-adrenoceptor subtypes (Fig. 4, A and B). Isoproterenol increased glucose uptake in BAT in vivo in FVB and ... WebCytochalasin B (1 mg; Sigma-Aldrich; C6762). Add 2 ml DMSO into a vial with 1 mg cytochalasin B (500 μg; 100× CB stock solution). Divide into small tubes (10–20 μl) and store at − 80 °C. • TSA (1 mg; Sigma-Aldrich; T8552).
WebNov 11, 2008 · Cytochalasin B (10 μmol/L) and phloretin (20 μmol/L) inhibited glucose uptake 85 ± 1% and 84 ± 18%, respectively. In contrast, the selective GLUT4 inhibitor …
WebCytochalasin B, CAS: 14930-96-2, is a cell-permeable fungal toxin used in actin polymerization studies and cytological research.. Cited in 16 publications ... Effect of cytochalasin B on glucose uptake, utilization, oxidation and insulinotropic action in tumoral insulin-producing cells. Malaisse, WJ., et al. 1987. Cell Biochem Funct. 5: 183-7. greentree animal clinic bridgeville paWebFeb 27, 2024 · The present study was conducted to explore the mechanism of nano-Zn absorption and its influence on lipid metabolism in the intestine of yellow catfish Pelteobagrus fulvidraco. Compared to ZnSO4, dietary nano-Zn addition increased the triglyceride (TG) content, enzymatic activities of malic enzyme (ME) and fatty acid … fnf christianCytochalasin B inhibits glucose transport [3] and platelet aggregation. It blocks adenosine -induced apoptotic body formation without affecting activation of endogenous ADP -ribosylation in leukemia HL-60 cells. [4] It is also used in cloning through nuclear transfer. See more Cytochalasin B, the name of which comes from the Greek cytos (cell) and chalasis (relaxation), is a cell-permeable mycotoxin. It was found that substoichiometric concentrations of cytochalasin B (CB) strongly inhibit … See more 1960s Cytochalasin B was first described in 1967, when it had been isolated from moulds by Dr W.B. Turner. Smith et al. found that CB … See more Cytochalasin B contains several highly polar keto- and hydroxyl groups and one peripheric lipophilic benzyl unit. See more There are ten possible sites for the in vitro degradation of cytochalasin B. There is not yet any evidence that the same sites are used for degradation in vivo, but evidence has confirmed the in vitro sites. Degradation is initialized by a periodate cleavage of the … See more Cytochalasins can be isolated from the fungi in which they naturally occur. Originally, they were isolated from Helminthosporium dematioideum. Other producers include See more It is suggested that the predominant mechanism of cytochalasin B is the inhibition of actin filament polymerization through binding to … See more Interactions When adding cytochalasin B and the beta-andrenergic agonist (-)-isoproterenol, prostaglandin E1 or cholera toxin to wild type S49 … See more green tree and landscapingWebMay 23, 2003 · Glucose levels dropped even in the presence of the transport inhibitor cytochalasin B, indicating rapid metabolism. Consistently, the addition of 2 … fnf christmas boyfriendWebMay 1, 1972 · Abstract. A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-d-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, … green tree and shrub careWebCytochalasin B has been shown to potently inhibit the transport of glucose, deoxyglucose, and glucosamine by Novikoff hepatoma cells in suspension culture without affecting their … fnf christmas hopeWebA mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy- d -glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is achieved at … greentree and park hill apartments wa