Cry2 mutant
WebMay 11, 2024 · First, in contrast to the control yellow fluorescent protein (YFP)–CRY2 that fully rescues the long hypocotyl phenotype of the cry1 … WebFeb 17, 2004 · Single, double, triple and quadruple mutants of phyA, phyB, cry1 and cry2 were exposed to different sunlight irradiances and photoperiods to investigate the roll played by phytochrome A, phytochrome B, cryptochrome 1 and cryptochrome 2 during de-etiolation of Arabidopsis thaliana seedlings under natural radiation. Even the quadruple mutant …
Cry2 mutant
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WebAug 16, 2016 · In contrast, mutant CRY2 was released less readily than CRY2-WT from the complexes even though both forms of CRY2 were bound to FBXL3 at the same level . In addition, we tested FBXL3-mediated CRY2 degradation using KL001, a small synthetic molecule known to stabilize CRY1 and CRY2 ( Hirota et al., 2012 ) due to its structural … WebMay 11, 2024 · Similar to the cry2 mutant 4, the ppk123 and ppk124 triple mutants and independent amiR 4k lines exhibited delayed flowering in long-day photoperiods (Fig. 8a–c, Supplementary Fig. 10), which is ...
WebThe mutations, cry2-1 and cry2-2, resulted from partial and complete deletion, respectively, of the CRY2 gene (Fig. 1C). Young seedlings of the cry2 mu-tants are impaired in blue-light–dependent hypocotyl inhibition and cotyledon open-ing (10), and cry2 mutant plants flowered later than normal (Fig. 1, E and F). The late-flowering phenotype ... WebJul 6, 2024 · Neither mutant affects steady-state levels of overexpressed c-MYC, and they have divergent impacts on circadian rhythms and on the ability of CRY2 to interact with SCF FBXL3 Unexpectedly, stable expression of either CRY2 D325H or of CRY2 S510L …
WebDec 12, 2024 · Plant cryptochromes (cry) are blue-light photoreceptors involved in multiple signaling pathways and various photomorphogenic responses. One biologically hyperactive mutant of a plant cryptochrome that was previously characterized is Arabidopsis cry1 L407F (Exner et al. in Plant Physiol 154:1633–1645, 2010). WebGenetic studies showed that mutation of TOE1 and TOE2 partially suppresses the late-flowering phenotype of cry1 cry2 mutant plants. BL-triggered interactions of CRY2 with TOE1 and TOE2 promote the dissociation of TOE1 and TOE2 from CO, resulting in alleviation of their inhibition of CO transcriptional activity and enhanced transcription of …
WebSep 15, 2024 · For each CRY2 mutant, the model with the lowest DOPE score is used for final visualization and electrostatic analysis. The distribution of electrostatic potential on the surface of each CRY2 mutant was calculated based on the Poisson–Boltzmann …
WebMar 7, 2024 · Cry2 exerts a critical role in the control of depression-related emotional states and modulates the chronobiological gene expression profile in the mouse amygdala. Cry1/Cry2-deficient mice had significantly lower N6- methyladenosine methylation of … dona pzn 04851108Webcrossed into a photoreceptor mutant background under different light conditions. The late-flowering phenotype of the phl-1 mutant was completely overridden by the phyB mutation (phl-1 vs. phyB phl-1)(Fig.1F and G). A similar strong dependency of cry2 on phyB has been reported (26). Hence, as is the case with cry2, PHL quiz z ortografii klasa 6WebThe activity of the Lhcb1*2 promoter was higher in the cry1 cry2 mutant than in the cry2 mutant, suggesting that cry1 could be involved in blue-light repression of photosynthetic genes. Surprisingly, the phyA cry1 cry2 triple mutant flowered earlier and showed better response to photoperiod than the cry1 cry2 double mutant, indicating that phyA ... quiz z ortografii klasa 3WebAug 23, 2024 · After analyzing phot1phot2, cry1 cry2, phot1phot2 cry1 cry2 mutants under low- high-intensity BL,we found phot1phot2 mutant showed slightrecovery phototropicresponse Figure hypocotylcurvature differentmutant seedlings Hypocotylphototropism 3-d-oldetiolated seedlings wildtype (WT) rpt2-2,nph3-6, phot1 … quizz openaskWebJan 26, 2007 · The CRY2-GFP gene was fused to these promoters and introduced into the cry2 mutant of Arabidopsis by the Agrobacterium tumefaciens–mediated method.As a control, the CRY2-GFP gene was expressed under the control of the authentic CRY2 promoter (Toth et al., 2001).We established several homozygous lines for each … quiz zoo animalsWebApr 15, 1999 · To explore the biological function of mammalian Cry1 and Cry2, we have generated cry1 and cry2 mutant mice through gene targeting in embryonic stem cells . Analysis ... quiz z ortografii klasa 5WebFeb 27, 1998 · We isolated two Arabidopsis mutant alleles that accumulate no CRY2 protein (Fig. 1A) ().The mutations, cry2-1 andcry2-2, resulted from partial and complete deletion, respectively, of the CRY2 gene (Fig. 1C). Young seedlings of the cry2 mutants … quizz otaku