WebJan 13, 2003 · SCAT3-expressing HeLa cells were exposed to 10 μg/ml CHX or 50 ng/ml TNF-α/10 μg/ml CHX for 6 h, then washed three times in PBS at 37°C. The cells were resuspended in PBS and used for fluorescence spectra determinations with an excitation wavelength of 435 nm at 37°C using a fluorescence spectrophotometer (model F2500; … WebSep 23, 2009 · HeLa cells overexpressing a kinase-deficient form of ERK-1 (K71R) were more sensitive to TNF and CHX. In the ERK-1 cells, Bad was phosphorylated during TNF + CHX treatment. In the HeLa wt cells and in the K71R clones TNF and CHX decreased Bad phosphorylation. ERK-1 cells treated with TNF and CHX did not release cytochrome c …
流感病毒诱导肿瘤细胞凋亡及其机制的研究 - 豆丁网
WebMar 12, 1999 · CHX-induced cell death was performed by incubating the cells in 20 μg/ml CHX for 6 h, and staurosporine (STS)-induced apoptosis was accomplished by incubating cells with various concentrations (0.1, 0.3, 0.5, 0.8, and 1.0 μ m) of STS for 4 h. WebFor research use only. Cycloheximide (NSC-185, Actidione, Naramycin A, CHX, FT 3422-2, NM-MCD 80), an antifungal antibiotic, is an eukaryote protein synthesis inhibitor with IC50 of 532.5 nM and 2880 nM for protein synthesis and RNA synthesis in vivo, respectively. Cycloheximide suppresses ferroptosis and inhibits autophagy. CAS No. 66-81-9 shannon construction
Ubiquitin-Dependent Degradation of Mitochondrial Proteins
WebJun 5, 2024 · HeLa cells were treated with MG132 or DMSO for 16 hr in the presence or absence of CHX. Levels of mitochondrial proteins were revealed by western blot of total … WebMurder for Hire Misguided Mom. A teenage mother starts to make questionable choices when the father of her two children comes back into their lives; when the court grants … WebCycloheximide solution Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture Synonym (s): CHX Empirical Formula (Hill Notation): C15H23NO4 CAS Number: 66-81-9 Molecular Weight: 281.35 MDL number: MFCD00082346 PubChem Substance ID: 24892732 NACRES: NA.77 Pricing and availability is not currently … polystorm recycled crate 1000x500x400 psm1a